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KMID : 0043319960190010012
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Archives of Pharmacal Research
1996 Volume.19 No. 1 p.12 ~ p.17
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Important Role of Glutathione in Protecting Against Menadione-Induced Cytotoxicity in Rat Platelets
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Cho Youn-Sook
Seung Sang-Ae
Kim Mee-Jeong
Lee Joo-Young
Chung Jin-Ho
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Abstract
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Our previous studies demonstrate that menadione (MEN) is cytotoxic to platelets of rats by depleting glutathione (GSH). In order to clarify whether GSH has a role in protecting against menadione-induced cytotoxicity, the effect of GSH depletors as well as GSH precusors on menadione-induced cytotoxicity was investigated. Cysteine and dithiothreitol (DTT) prevent MEN-induced cytotoxicity in a dose-dependent manner, as determined by LDH leakage and change in turbidity. When platelets were treated with 1-chloro-2,4-dinitrobenzene (CDNB) and diethylmaleate (DEM), both of which deplete intracellular GSH, MEN-induced cytotoxicity was potentiated in the CDNB-treated paltelets, but not in the DEM-treated platelets. These data suggest that the GSH in platelets plays an important role in protecting against cytotoxicity induced by menadione.
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KEYWORD
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Menadione, Platelets, Glutathione, Cytotoxicity
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